Aja, Patrick M.Chukwu, Chinecherem A.Paul-Chima, Ugwu O.Ale, Boniface A.Agu, Peter C.Deusdedi, TusubiraChukwu, Darlington C.Ani, Onyedika G.Ekpono, Ezebuilo U.Ogwoni, Hilary A.Awoke, Joshua N.Ogbu, Patience N.Aja, LucyUgochukwu, Oliver U.Uche Orji, ObasiPeter Nweke, ChinosoEgwu, ChineduEkpono, Ejike U.Onyinyechi Ewa, GiftIgwenyi, Ikechuku O.Ugo Alum, EstherEjim Uti, DanielEmeka Offor, ChristianIfie, Josiah E.Njoku, AmaobichukwuEkenechukwu Kenneth, MaduagwunnaDaniel Eze, Ejike2023-10-292023-10-292023-10-20Aja, P. M., Chukwu, C. A., Paul-Chima, U. O., Ale, B. A., Agu, P. C., Deusdedi, T., Chukwu, D. C., Ani, O. G., Ekpono, E. U., Ogwoni, H. A., Awoke, J. N., Ogbu, P. N., Aja, L., Ugochukwu, O. U., Uche Orji, O., Peter Nweke, C., Egwu, C., Ekpono, E. U., Onyinyechi Ewa, G., Igwenyi, I. O., ... Daniel Eze, E. (2023). Cucumeropsis mannii seed oil protects against bisphenol A-induced hepatotoxicity by mitigating inflammation and oxidative stress in rats. RPS Pharmacy and Pharmacology Reports, 3(1), article-number rqad033. https://doi.org/10.1093/rpsppr/rqad03310.1093/rpsppr/rqad033http://hdl.handle.net/10034/628237© The Author(s) 2023. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society.OBJECTIVES This study looked at how CMSO affected male Wistar albino rats' liver damage caused by bisphenol A. METHODS The standard HPLC method was used to assess the CMSO's phenolic content. Then, six (n = 8) groups of forty-eight (48) male Wistar rats (150 20 g) each received either CMSO or olive oil before being exposed to BPA for 42 days. Groups: A (one milliliter of olive oil, regardless of weight), B (BPA 100 mg/kg body weight (BW)), C (CMSO 7.5 mg/kg BW), D (CMSO 7.5 mg/kg BW + BPA 100 mg/kg BW), E (CMSO 5.0 mg/kg BW + BPA 100 mg/kg BW), and F (CMSO 2.5 mg/kg BW + BPA 100 mg/kg BW). KEY FINDINGS A surprising abundance of flavonoids, totaling 17.8006 10.95 g/100 g, were found in the HPLC data. Malondialdehyde, liver enzymes, reactive oxygen species, total bilirubin, and direct bilirubin levels were all significantly elevated by BPA (p 0.05). Additionally, nuclear factor-B, interleukin-6, interleukin-1, tumor necrosis factor, and histological alterations were all considerably (p 0.05) caused by BPA. The altered biochemical markers and histology were, however, noticeably recovered by CMSO to a level that was comparable to the control. CONCLUSION Due to the abundance of flavonoid components in the oil, CMSO protects the liver from BPA-induced hepatotoxicity by lowering oxidative stress and inflammatory reactions.Licence for this article: https://creativecommons.org/licenses/by/4.0/https://creativecommons.org/licenses/by/4.0/Article2754-5849RPS Pharmacy and Pharmacology Reports2023-10-29