Akpodiete, Nwamaka O.Carlos, BiancaVoges, KamilaNunes, Bruno TinocoSouza-Neto, Jayme AugustusNoulin, FlorianTonge, DanielZuharah, Wan FatmaTripet, Frédéric2025-05-132025-05-132025-05-05Akpodiete, N. O., Carlos, B., Voges, K., Nunes, B. T., Souza-Neto, J. A., Noulin, F., Tonge, D., Zuharah, W. F. & Tripet, F. (2025). Improvement of water quality for mass anopheline rearing: Dynamics of larval tray bacterial communities under different water treatments revealed by 16S ribosomal RNA amplicon sequencing. Journal of Applied Microbiology, 136(5), article-number lxaf110. https://doi.org/10.1093/jambio/lxaf1101364-507210.1093/jambio/lxaf110http://hdl.handle.net/10034/629407© The Author(s) 2025. Published by Oxford University Press on behalf of Applied Microbiology International.BACKGROUND: Immature anophelines inhabit aquatic environments with diverse physicochemical properties and microorganisms. In insectary settings, ammonia accumulation in larval rearing trays can lead to high larval mortality. Bacterial communities in these trays may influence ammonia levels through nitrification and denitrification. While symbiotic bacteria are known to be crucial for nutrition, digestion, reproduction, and immune responses in anophelines, the microbial communities specifically associated with Anopheles coluzzii larvae have not been characterised. METHODS AND RESULTS: Building on a study examining ammonia-capturing zeolite and water changes for rearing Anopheles coluzzii, this research characterised the bacterial communities using 16S rRNA gene sequencing to identify species linked to larval survival and phenotypic quality. Functional filters were applied to identify bacteria related to ammonia nitrification and their impact on larval development. qPCR was used to validate the sequencing data for the ten most significant bacteria. Water changes significantly reduced bacterial diversity and abundance, improving adult mosquito development and quality. In contrast, untreated trays showed a higher abundance of potentially harmful bacteria, adversely affecting development. Applying zeolite increased nitrifying bacteria presence, benefiting mosquito growth while lowering toxic bacteria levels-trends confirmed by qPCR. CONCLUSIONS: This study offers insights into the bacterial communities in mosquito larval-rearing water, highlighting species that could enhance ammonia nitrification and overall rearing success.enhttps://creativecommons.org/licenses/by/4.0/Infectious DiseasesGeneticsAnopheles coluzziiBacterial communitiesMosquitosArticle1365-2672Journal of Applied Microbiology2025-05-13136