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Alignment of multiple glial cell populations in 3D nanofiber scaffolds: toward the development of multicellular implantable scaffolds for repair of neural injury

Weightman, Alan P.
; 
Jenkins, Stuart I.
; 
Pickard, Mark R.
; 
Chari, Divya M.
; 
Yang, Ying
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Affiliation
Keele University, United Kingdom
EPub Date
Publication Date
2014-02
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Chester Medical School
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Abstract
Non-neuronal cells of the central nervous system (CNS), termed "neuroglia," play critical roles in neural regeneration; therefore, replacement of glial populations via implantable nanofabricated devices (providing a growth-permissive niche) is a promising strategy to enhance repair. Most constructs developed to date have lacked three-dimensionality, multiple glial populations and control over spatial orientations, limiting their ability to mimic in vivo neurocytoarchitecture. We describe a facile technique to incorporate multiple glial cell populations [astrocytes, oligodendrocyte precursor cells (OPCs) and oligodendrocytes] within a three-dimensional (3D) nanofabricated construct. Highly aligned nanofibers could induce elongation of astrocytes, while OPC survival, elongation and maturation required pre-aligned astrocytes. The potential to scale-up the numbers of constituent nanofiber layers is demonstrated with astrocytes. Such complex implantable constructs with multiple glial sub-populations in defined 3D orientations could represent an effective approach to reconstruct glial circuitry in neural injury sites.
Citation
Weightman, A., Jenkins, S., Pickard, m., Chari, D., & Yang, Y. (2014). Alignment of multiple glial cell populations in 3D nanofiber scaffolds: toward the development of multicellular implantable scaffolds for repair of neural injury. Nanomedicine: Nanotechnology, Biology and Medicine, 10(2), 291-95. http://dx.doi.org/10.1016/j.nano.2013.09.001
Publisher
Elsevier
Journal
Nanomedicine: Nanotechnology, Biology and Medicine
Research Unit
URI
http://hdl.handle.net/10034/606276
DOI
10.1016/j.nano.2013.09.001
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Article
Language
en
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ISSN
1549-9634
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http://www.journals.elsevier.com/nanomedicine-nanotechnology-biology-and-medicine