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Antioxidant, Anticancer and Antimicrobial, Effects of Rubia cordifolia Aqueous Root Extract
Barlow, R. Barnes, D. A. Campbell, Anna M. Nigam, Poonam S. Owusu-Apenten, Richard K.
Barlow, R.
Barnes, D. A.
Campbell, Anna M.
Nigam, Poonam S.
Owusu-Apenten, Richard K.
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Publication Date
2015-11-10
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Abstract
Aims: To evaluate the total antioxidant capacity (TAC) of Rubia cordifolia root extracts, to test anticancer activity against MDA-MB-231 breast cancer cell lines, and to evaluate antimicrobial activity of the same extract versus six Gram-positive and negative bacteria.
Study Design: In vitro.
Place of Study and Duration: School of Biomedical Sciences, Ulster University, July 2014-Sept 2015.
Methodology: TAC was tested using ABTS, DPPH, FRAP and Folin assays and values were expressed as mg-gallic acid equivalents per 100 g (GAE/100 g) of sample. Anticancer properties were examined against MDA-MB-231 breast cancer cell lines using Sulforhodamine B assay. Antimicrobial activity was examined using a disk diffusion assay with three Gram-positive (Staphylococcus epidermidis, Staphylococcus aureus and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi) bacteria.
Results: TAC of dry extracts of Rubia cordifolia ranged from 523±43 to 4513±208 (mg GAE mg/100 g) depending on the method of analysis, ABTS> FRAP> Folin > DPPH methods. R. cordifolia dry extract showed cytotoxicity against MDA-MB-231 with IC50 = 44 µg/ml or 5.1µM GAE. No antimicrobial activity was observed against the three Gram-positive, or three Gram-negative bacterial species using the water extract or R. cordifolia.
Conclusion: R. cordifolia aqueous extract possess high total antioxidant capacity but values depend on the method of analysis. R. cordifolia extract inhibits MDA-MB-231 breast cancer cells proliferation but nil anti-bacterial activity was observed for three Gram-positive and three Gram-negative bacterial strains tested.
Citation
Barlow, R., Barnes, D., Campbell, A., Nigam, P. S., & Owusu-Apenten, R. (2015). Antioxidant, anticancer and antimicrobial, effects of Rubia cordifolia aqueous root extract. Journal of Advances in Biology & Biotechnology, 5(1), 6-14.
Publisher
SCIENCEDOMAIN International
Journal
Journal of Advances in Biology & Biotechnology
Research Unit
DOI
10.9734/JABB/2016/22625
PubMed ID
PubMed Central ID
Type
Article
Language
en
Description
Received 15th October 2015
Accepted 29th October 2015
Published 10th November 2015
Series/Report no.
ISSN
EISSN
2394-1081