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Mesenchymal stem cell conditioned medium increases glial reactivity and decreases neuronal survival in spinal cord slice cultures
Wood, Chelsea R. Juárez, Esri H. Ferrini, Francesco Myint, Peter Innes, John Lossi, Laura Merighi, Adalberto Johnson, William E. B.
Wood, Chelsea R.
Juárez, Esri H.
Ferrini, Francesco
Myint, Peter
Innes, John
Lossi, Laura
Merighi, Adalberto
Johnson, William E. B.
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EPub Date
Publication Date
2021-03-03
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Abstract
Spinal cord slice cultures (SCSC) allow study of spinal cord circuitry, maintaining stimuli responses comparable to live animals. Previously, we have shown that mesenchymal stem/stromal cell (MSC) transplantation reduced inflammation and increased nerve regeneration but MSC survival was short-lived, highlighting that beneficial action may derive from the secretome. Previous studies of MSC conditioned medium (CM) have also shown increased neuronal growth. In this study, murine SCSC were cultured in canine MSC CM (harvested from the adipose tissue of excised inguinal fat) and cell phenotypes analysed via immunohistochemistry and confocal microscopy. SCSC in MSC CM displayed enhanced viability after propidium iodide staining. GFAP immunoreactivity was significantly increased in SCSC in MSC CM compared to controls, but with no change in proteoglycan (NG2) immunoreactivity. In contrast, culture in MSC CM significantly decreased the prevalence of βIII-tubulin immunoreactive neurites, whilst Ca transients per cell were significantly increased. These results contradict previous and reports of how MSC and their secretome may affect the microenvironment of the spinal cord after injury and highlight the importance of a careful comparison of the different experimental conditions used to assess the potential of cell therapies for the treatment of spinal cord injury. [Abstract copyright: © 2021 Published by Elsevier B.V.]
Citation
Wood, C. R., Juárez, E. H., Ferrini, F., Myint, P., Innes, J., Lossi, L., Merighi, A., & Johnson, W. E. B. (2021). Mesenchymal stem cell conditioned medium increases glial reactivity and decreases neuronal survival in spinal cord slice cultures. Biochemistry and Biophysics Reports, 26, article-number 100976. https://doi.org/10.1016/j.bbrep.2021.100976
Publisher
Elsevier
Journal
Biochemistry and Biophysics Reports
Research Unit
DOI
10.1016/j.bbrep.2021.100976
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PubMed Central ID
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Article
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Series/Report no.
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EISSN
2405-5808