Application of immunological methods for the detection of species adulteration in dairy products
Hurley, Ian P. Ireland, H. Elyse Coleman, Robert C. Williams, John H. H.
Hurley, Ian P.
Ireland, H. Elyse
Coleman, Robert C.
Williams, John H. H.
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EPub Date
Publication Date
2004-10-20
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Abstract
A number of enzyme-linked immunosorbent assays (ELISAs) have been developed for the detection of milk adulteration in dairy products. Target antigens have been caseins, lactoglobulins, immunoglobulins and other whey proteins. Polyclonal and monoclonal antibodies have been used in a variety of formats including direct, indirect, competitive and sandwich ELISAs. ELISAs have been successfully applied to the detection of cows' milk adulteration of sheep, goat and buffalo milk. Goat milk adulteration of sheep milk has also been detected. A number of ELISAs have also been applied to cheese. It is recommended that ELISA should be used in combination with PCR to ensure compliance with current legislation.
Citation
Hurley, I. P., Ireland, H. E., Coleman, R. C., & Williams, J. H. H. (2004). Application of immunological methods for the detection of species adulteration in dairy products. International Journal of Food Science + Technology, 39(8), 873-878. https://doi.org/10.1111/j.1365-2621.2004.00861.x
Publisher
Wiley
Journal
International Journal of Food Science + Technology
Research Unit
DOI
10.1111/j.1365-2621.2004.00861.x
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PubMed Central ID
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Article
Language
en
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ISSN
0950-5423
EISSN
1365-2621