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Antioxidant, Anticancer and Antibacterial Activity of Withania somnifera Aqueous Root Extract

Barnes, D. A.
; 
Barlow, R.
; 
Nigam, Poonam S.
; 
Owusu-Apenten, Richard K.
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University of Chester, University of Ulster
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2015-11-10
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Clinical Sciences and Nutrition
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Abstract
Aims: To evaluate total antioxidant capacity, anticancer activity and antibacterial effects Withania somnifera aqueous-root extracts. Study Design: In vitro study. Place of Study: School of Biomedical Sciences, Ulster University, UK. Methodology: Total antioxidant capacity (TAC) of whole powder and freeze dried W. somnifera aqueous-root extracts was determined using FRAP, DPPH, Folin and ABTS assays. Anticancer activity was accessed using MDA-MB-231 breast cells and Sulforhodamine B staining for cell viability. Antibacterial activity was by disk diffusion assay with penicillin, amoxicillin and streptomycin as positive controls. Results: The TAC for W. somnifera extract was 86, 47, 195,or 443 gallic acid equivalents per 100g dry basis (mgGAE/ 100 g) using FRAP, DPPH, Folin or ABTS assays, respectively. Corresponding TAC values for freeze dried W. somnifera aqueous-root extract were, 418, 553, 1898 or, 1770 (mgGAE/100 g). W. somnifera aqueous-root extract inhibited MDA-MB-231 cell proliferation in a dose-dependent manner with IC50 = 0.19 mg/ml (21 µM GAE). Nil antibacterial effects were detected for freeze dried W. somnifera extract (0-1 mg/ml) across six species of bacteria tested. Conclusion: Withania somnifera root water extract showed significant antioxidant and anticancer activity for MDA-MB-231 breast cancer cells but no antibacterial activity under the conditions of this study.
Citation
Barnes, D. A., Barlow, R., Nigam, P. S., & Owusu-Apenten, R. (2015). Antioxidant, Anticancer and Antibacterial Activity of Withania somnifera Aqueous Root Extract. Journal of Advances in Biology & Biotechnology, 5(1), 1-6.
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SCIENCEDOMAIN International
Journal
Journal of Advances in Biology & Biotechnology
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URI
http://hdl.handle.net/10034/621908
DOI
10.9734/JABB/2016/22523
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Article
Language
en
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2394-1081
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http://www.sciencedomain.org/abstract/12257