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SP3 protocol optimised for foodcrust protein extraction from archaeological cooking vessels

Harvey, Virginia L.
; 
Hendy, Jessica
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Affiliation
University of Chester; University of York
EPub Date
Publication Date
2025-01-30
Submitted Date
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Biological Sciences
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Abstract
A SP3-based protocol developed for the extraction of proteins from charred organic residues (foodcrust) found on cooking vessels. This protocol is to prepare samples for MALDI-ToF MS and LC-MS/MS analysis. We recommend starting with a batch size of 2-10 samples, and including flanking negative controls (blanks) to test for any downstream contamination.
Citation
Harvey, V.L. & Hendy, J. 2025. SP3 protocol optimised for foodcrust protein extraction from archaeological cooking vessels. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvwb22wvmk/v1
Publisher
Springer Nature
Journal
protocols.io
Research Unit
URI
http://hdl.handle.net/10034/629230
DOI
10.17504/protocols.io.e6nvwb22wvmk/v1
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PubMed Central ID
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Other
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Series/Report no.
ISSN
1754-2189
EISSN
1750-2799
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Sponsors
The Leverhulme Trust
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https://www.protocols.io/view/sp3-protocol-optimised-for-foodcrust-protein-extra-e6nvwb22wvmk/v1/metadata