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CD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic than plastic adherent mesenchymal stem cells

Kohli, Nupur
; 
Johnson, William E. B.
; 
Uchida, Kenzo
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Aston University; University of Chester; University of Fukui
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2019-02-28
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Chester Medical School
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Abstract
CD271 is a marker of bone marrow MSCs with enhanced differentiation capacity for bone or cartilage repair. However, the nature of CD271+ MSCs from adipose tissue (AT) is less well understood. Here, we investigated the differentiation, wound healing and angiogenic capacity of plastic adherent MSCs (PA MSCs) versus CD271+ MSCs from AT. There was no difference in the extent to which PA MSCs and CD271+ MSCs formed osteoblasts, adipocytes or chondrocytes in vitro. In contrast, CD271+ MSCs transplanted into athymic rats significantly enhanced osteochondral wound healing with reduced vascularisation in the repair tissue compared to PA MSCs and control animals; there was little histological evidence of mature articular cartilage formation in all animals. Conditioned medium from CD271+ MSC cultures was less angiogenic than PA MSC conditioned medium, and had little effect on endothelial cell migration or endothelial tubule formation in vitro. The low angiogenic activity of CD271+ MSCs and improved early stage tissue repair of osteochondral lesions when transplanted, along with a comparable differentiation capacity along mesenchymal lineages when induced, suggests that these selected cells are a better candidate than PA MSCs for the repair of cartilaginous tissue.
Citation
Kohli, N., Al-Delfi, I. R., Snow, M., Sakamoto, T., Miyazaki, T., Nakajima, H., ... & Johnson, W. E. (2019). CD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic than plastic adherent mesenchymal stem cells. Scientific Reports, 9, article-number 3194. https://doi.org/10.1038/s41598-019-39715-z
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Nature Research
Journal
Scientific Reports
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http://hdl.handle.net/10034/623519
DOI
10.1038/s41598-019-39715-z
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Article
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The version of record of this article, first published in [Scientific Reports], is available online at Publisher’s website: http://dx.doi.org/10.1038/s41598-019-39715-z
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2045-2322
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https://www.nature.com/articles/s41598-019-39715-z