Loading...
Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract.
Chau, Tsz Ching Owusu-Apenten, Richard K. Nigam, Poonam S.
Chau, Tsz Ching
Owusu-Apenten, Richard K.
Nigam, Poonam S.
Advisors
Editors
Other Contributors
Affiliation
EPub Date
Publication Date
2017-10-28
Submitted Date
Collections
Files
Loading...
Main article
Adobe PDF, 107.96 KB
Other Titles
Abstract
Aims: To evaluate total phenols content (TPC), antioxidant capacity (TAC) and antibacterial activity of Manuka honey extract (MHE) and to compare such properties with those for unfractionated Manuka honey. Study Design: In vitro study. Place and Duration of Study: School of Biomedical Sciences, Ulster University, Coleraine, UK. Between September 2016 and September 2017. Methodology: MHE was prepared by solvent extraction using ethyl acetate. TPC was determined by Folin-Ciocalteu assay. The iron (III) reducing antioxidant capacity (IRAC) method was used to determine TAC. Antibacterial activity was evaluated using disc diffusion assay and 96-well microtiter plate methods with absorbance measured at 600 nm. Results: The TPC for MHE was 30-fold higher than the value for Manuka honey (33420±1685 mg vs. 1018±78 mg GAE/kg) while TAC values were~ 100-times greater (83,198±7064 vs. 793±104 TEAC, respectively). Antibacterial activity assessed by disc diffusion for Manuka honey (18.5 mm on S. aureus and 20 mm on E. coli) was two times greater than for MHE (9mm for both S. aureus and E. coli). The 96-well microtiter plate assay confirmed the greater antibacterial activity for Manuka
Citation
Chau, T. C., Owusu-Apenten, R., & Nigam, P. S. (2017). Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract. J Adv Biol Biotech, 15, 1-6.
Publisher
SCIENCEDOMAIN International
Journal
Journal of Advances in Biology & Biotechnology
Research Unit
DOI
10.9734/JABB/2017/37101
PubMed ID
PubMed Central ID
Type
Article
Language
en
Description
Series/Report no.
ISSN
EISSN
2394-1081