Loading...
Fgf signalling triggers an intrinsic mesodermal timer that determines the duration of limb patterning
Sedas Peres, Sofia McQueen, Caitlin Stainton, Holly Pickering, Joseph Chinnaiya, Kavitha Saiz Lopez, Patricia Placzek, Marysia Ros, Maria Towers, Matthew
Sedas Peres, Sofia
McQueen, Caitlin
Stainton, Holly
Pickering, Joseph
Chinnaiya, Kavitha
Saiz Lopez, Patricia
Placzek, Marysia
Ros, Maria
Towers, Matthew
Advisors
Editors
Other Contributors
EPub Date
Publication Date
2023-09-20
Submitted Date
Collections
Files
Loading...
Article - VoR
Adobe PDF, 3.97 MB
Other Titles
Abstract
Complex signalling between the apical ectodermal ridge (AER - a thickening of the distal epithelium) and the mesoderm controls limb patterning along the proximo-distal axis (humerus to digits). However, the essential in vivo requirement for AER-Fgf signalling makes it difficult to understand the exact roles that it fulfils. To overcome this barrier, we developed an amenable ex vivo chick wing tissue explant system that faithfully replicates in vivo parameters. Using inhibition experiments and RNA-sequencing, we identify a transient role for Fgfs in triggering the distal patterning phase. Fgfs are then dispensable for the maintenance of an intrinsic mesodermal transcriptome, which controls proliferation/differentiation timing and the duration of patterning. We also uncover additional roles for Fgf signalling in maintaining AER-related gene expression and in suppressing myogenesis. We describe a simple logic for limb patterning duration, which is potentially applicable to other systems, including the main body axis.
Citation
Sedas Peres, S., McQueen, C., Stainton, H., Pickering, J., Chinnaiya, K., Saiz Lopez, P., Placzek, M., Ros, M., & Towers, M. (2023). Fgf signalling triggers an intrinsic mesodermal timer that determines the duration of limb patterning. Nature Communications, 14, 5841. https://doi.org/10.1038/s41467-023-41457-6
Publisher
Nature Research
Journal
Nature Communications
Research Unit
DOI
10.1038/s41467-023-41457-6
PubMed ID
PubMed Central ID
Type
Article
Language
Description
Series/Report no.
ISSN
EISSN
2041-1723