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The influence of pH and fluid dynamics on the antibacterial efficacy of 45S5 Bioglass Short title: Antibacterial efficacy of 45S5 Bioglass
Begum, Saima Johnson, William E. B. Worthington, Tony Martin, Richard
Begum, Saima
Johnson, William E. B.
Worthington, Tony
Martin, Richard
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2016-02-02
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Abstract
In recent years, there has been considerable interest in the potential antibacterial properties that bioactive glasses may possess. However, there have been several conflicting reports on the antibacterial efficacy of 45S5 Bioglass®. Various mechanisms regarding its mode of action have been proposed, such as changes in the environmental pH, increased osmotic pressure, and 'needle-like' sharp glass debris which could potentially damage prokaryotic cell walls and thus inactivate bacteria. In this current study, a systematic investigation was undertaken on the antibacterial efficacy of 45S5 Bioglass® on Escherichia coli NCTC 10538 and Staphylococcus aureus ATCO 6538 under a range of clinically relevant scenarios including varying Bioglass® concentration, direct and indirect contact between Bioglass® and microorganisms, static and shaking incubation conditions, elevated and neutralised pH environments. The results demonstrated that, under elevated pH conditions, Bioglass® particles have no antibacterial effect on S. aureus while a concentration dependent antibacterial effect against E. coli was observed. However, the antibacterial activity ceased when the pH of the media was neutralised. The results of this current study, therefore, suggest that the mechanism of antibacterial activity of Bioglass® is associated with changes in the environmental pH; an environment that is less likely to occur in vivo due to buffering of the system.
Citation
Begum, S., Johnson, W. E., Worthington, T., & Martin, R. A. (2016). The influence of pH and fluid dynamics on the antibacterial efficacy of 45S5 Bioglass. Biomedical Materials, 11(1), 015006.
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IOP Publishing
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Biomedical Materials
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DOI
10.1088/1748-6041/11/1/015006
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Article
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This is an author-created, un-copyedited version of an article accepted for publication/published
in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or
any version derived from it. The Version of Record is available online at https://doi.org/10.1088/1748-6041/11/1/015006
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1748-605X