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Chromosome segregation in Escherichia coli involves DNA binding by the MinC-MinD complex

Palanisamy, Navaneethan
; 
Klauss, Linda
; 
Lefebvre, Maïween
; 
Liang, Jungsheng
; 
Hofhaus, Götz
; 
Zeelen, Johan
; 
Urban, Maximilian
; 
Öztürk, Mehmet Ali
; 
Merker, Sabine
; 
Luzarowski, Marcin
... show 2 more
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Affiliation
University of Freiburg; University of Heidelberg; Ludwig-Maximilians-Universität München; University of Chester; Gyros Protein Technologies AB
EPub Date
Publication Date
2025-02-06
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Chester Medical School
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Abstract
The Escherichia coli Min system, composed of the proteins MinC, MinD, and MinE, is crucial for directing septum formation at mid-cell. MinD has also been implicated in chromosome segregation due to its non-sequence-specific DNA binding ability. Here, we demonstrate that the MinCD complex, rather than MinD alone, is the primary driver of Min-mediated chromosome segregation and that MinE regulates the transient nature of this interaction. We further reveal a critical function for the linker connecting MinC’s N- and C-terminal domains in both DNA binding and FtsZ polymerization inhibition. E. coli strains expressing MinC linker variants from the native locus exhibit chromosome segregation defects. The defects are more pronounced in strains where the nucleoid-associated protein HU is endogenously tagged with GFP. These findings reinforce the involvement of the Min system in chromosome segregation in E. coli and highlight a previously unrecognized role for the MinC linker.
Citation
Palanisamy, N., Klauss, L., Lefebvre, M., Liang, J., Hofhaus, G., Zeelen, J., Urban, M., Öztürk, M. A., Merker, S., Luzarowski, M., & Di Ventura, B. (2025). Chromosome segregation in Escherichia coli involves DNA binding by the MinC-MinD complex. bioRxiv. https://doi.org/10.1101/2025.02.06.636808
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OpenRxiv (bioRxiv)
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URI
http://hdl.handle.net/10034/629464
DOI
10.1101/2025.02.06.636808
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Preprint
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ISSN
2692-8205
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https://www.biorxiv.org/content/10.1101/2025.02.06.636808v1