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Measuring the secretion of heat shock proteins from cells
Ireland, H. Elyse Leoni, Francesca Altaie, Ala Birch, Catherine S. Coleman, Robert C. Hunter-Lavin, Claire Williams, John H. H.
Ireland, H. Elyse
Leoni, Francesca
Altaie, Ala
Birch, Catherine S.
Coleman, Robert C.
Hunter-Lavin, Claire
Williams, John H. H.
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Editors
Other Contributors
Affiliation
EPub Date
Publication Date
2007-10-03
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Abstract
This article outlines procedures, using Hsp70 as the example, to: ensure the status of cells (viable, apoptotic or necrotic); identify the heat shock protein secreted; and quantify the secreted protein. Hsp70 has previously been quantified by ELISA, but newer methods are now being adopted, such as BIAcore and bead-based assays for use by FACS. These methods have the advantages of being more sensitive and requiring less sample than ELISA. The BIAcore has the potential to analyse Hsp70 ligands and provide affinity constants.
Citation
Ireland, H. E., Leoni, F., Altaie, A., Birch, C. S., Coleman, R. C., Hunter-Lavin, C., & Williams, J H. H. (2007). Measuring the secretion of heat shock proteins from cells. Methods, 43(3), 176-183. https://doi.org/10.1016/j.ymeth.2007.06.011
Publisher
Elsevier
Journal
Methods
Research Unit
DOI
10.1016/j.ymeth.2007.06.011
PubMed ID
PubMed Central ID
Type
Article
Language
en
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This article is not available through ChesterRep.
Series/Report no.
ISSN
1046-2023