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A novel model of nephrotic syndrome results from a point mutation in Lama5 and is modified by genetic background
Falcone, Sara Nicol, Thomas Blease, Andrew Randles, Michael Angus, Elizabeth Page, Anton Tam, Frederick W. K. Pusey, Charles D. Lennon, Rachel Potter, Paul
Falcone, Sara
Nicol, Thomas
Blease, Andrew
Randles, Michael
Angus, Elizabeth
Page, Anton
Tam, Frederick W. K.
Pusey, Charles D.
Lennon, Rachel
Potter, Paul
Advisors
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Other Contributors
EPub Date
Publication Date
2021-11-10
Submitted Date
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Abstract
Nephrotic syndrome is characterized by severe proteinuria, hypoalbuminaemia, edema and hyperlipidaemia. Genetic studies of nephrotic syndrome have led to the identification of proteins playing a crucial role in slit diaphragm signaling, regulation of actin cytoskeleton dynamics and cell-matrix interactions. The laminin α5 chain is essential for embryonic development and, in association with laminin β2 and laminin γ1, is a major component of the glomerular basement membrane, a critical component of the glomerular filtration barrier. Mutations in LAMA5 were recently identified in children with nephrotic syndrome. Here, we have identified a novel missense mutation (E884G) in the uncharacterized L4a domain of LAMA5 where homozygous mice develop nephrotic syndrome with severe proteinuria with histological and ultrastructural changes in the glomerulus mimicking the progression seen in most patients. The levels of LAMA5 are reduced in vivo and the assembly of the laminin 521 heterotrimer significantly reduced in vitro. Proteomic analysis of the glomerular extracellular fraction revealed changes in the matrix composition. Importantly, the genetic background of the mice had a significant effect on aspects of disease progression from proteinuria to changes in podocyte morphology. Thus, our novel model will provide insights into pathologic mechanisms of nephrotic syndrome and pathways that influence the response to a dysfunctional glomerular basement membrane that may be important in a range of kidney diseases.
Citation
Falcone, S., Nicol, T., Blease, A., Randles, M. J., Angus, E., Page, A., Tam, F. W. K., Pusey, C. D., Lennon, R., & Potter, P. K. (2022). A novel model of nephrotic syndrome results from a point mutation in Lama5 and is modified by genetic background. Kidney International, 101(3), 527-540. https://doi.org/10.1016/j.kint.2021.10.031
Publisher
Elsevier
Journal
Kidney International
Research Unit
DOI
10.1016/j.kint.2021.10.031
PubMed ID
PubMed Central ID
Type
Article
Language
Description
Series/Report no.
ISSN
0085-2538
EISSN
1523-1755