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In vitro cyto-toxic assessment of heavy metals and their binary mixtures on mast cell-like, rat basophilic leukemia (RBL-2H3) cells
Eze, Chukwuebuka T. Michelangeli, Francesco Otitoloju, Adebayo A.
Eze, Chukwuebuka T.
Michelangeli, Francesco
Otitoloju, Adebayo A.
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EPub Date
Publication Date
2019-02-11
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Abstract
We investigated the cytotoxicity and mechanisms of cell death induced by salts of Cadmium (Cd2+), Lead
(Pb2+), Arsenic (AsO4
3−) and Chromium (Cr+6) on RBL-2H3 cells (a model mast cell line). In addition,
cyto-toxic effect on cell viability was assessed to reveal their nature of interaction in binary mixture.
The individual cytotoxic characteristics of these metals on RBL-2H3 cell viability showed a concentration-
dependent reduction of cell viability. We observed that concentration-dependent cytotoxic potency on
RBL-2H3 cells of these metals range in the following order Cd2+>Cr+6>As O4
3−>Pb2+ with LC50 values of
0.11 μM, 93.58 μM, 397.9 μM and 485.3 μM respectively. Additive effects were observed with Pb2+ + Cd2+,
Pb2+ + AsO4
3−, Pb2+ + Cr+6 and AsO4
3− + Cr+6. The study revealed that Pb2+, Cd2+, AsO4
3− and Cr+6 could
induce significant (P<0.01) cell death by apoptosis in RBL-2H3. Highly significant necrotic cell death was
observed with Pb2+ and Cr+6 (P<0.01) than Cd2+ and AsO4
3− (P<0.05). Overall, it can be deduced that several
cell death executing pathways may be concomitantly activated on exposure to heavy metals and the predominance
of one over others might depend on the type of heavy metal, concentration and the metabolic state of
the cell. Eventually, binary mixtures of some of these metals showed less cytotoxicity than would be expected
from their individual actions and may depend on the co-exposure of the metal ions and their modes of action.
Citation
Eze, C. T., Michelangeli, F. & Otitoloju, A. A. (2019). In vitro cyto-toxic assessment of heavy metals and their binary mixtures on mast cell-like, rat basophilic leukemia (RBL-2H3) cells. Chemosphere, 223, 686-93.
Publisher
Elsevier
Journal
Chemosphere
Research Unit
DOI
10.1016/j.chemosphere.2019.02.035
PubMed ID
PubMed Central ID
Type
Article
Language
en
Description
Series/Report no.
ISSN
0045-6535
EISSN
1879-1298