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Extracellular vesicles derived from umbilical cord mesenchymal stromal cells show enhanced anti-inflammatory properties via upregulation of miRNAs after pro-inflammatory priming
Hyland, Mairead Mennan, Claire Davies, Rebecca Wilson, Emma Tonge, Daniel P. Clayton, Aled Kehoe, Oksana
Hyland, Mairead
Mennan, Claire
Davies, Rebecca
Wilson, Emma
Tonge, Daniel P.
Clayton, Aled
Kehoe, Oksana
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EPub Date
Publication Date
2023-07-20
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Abstract
Autoimmune conditions, such as rheumatoid arthritis, are characterised by a loss of immune tolerance, whereby the immune cells attack self-antigens causing pain and inflammation. These conditions can be brought into remission using pharmaceutical treatments, but often have adverse side effects and some patients do not respond favourably to them. Human umbilical cord mesenchymal stromal cells (UCMSCs) present a promising alternative therapeutic due to their innate anti-inflammatory properties which can be strengthened using pro-inflammatory conditions. Their therapeutic mechanism of action has been attributed to paracrine signalling, by which nanosized acellular particles called 'extracellular vesicles' (EVs) are one of the essential components. Therefore, this research analysed the anti-inflammatory properties of UCMSC-EVs 'primed' with pro-inflammatory cytokines and at baseline with no inflammatory cytokines (control). Both control and primed EVs were co-cultured with un-pooled peripheral blood mononuclear cells (PBMCs; n = 6) from healthy donors. Neither control nor primed EVs exerted a pro-inflammatory effect on PBMCs. Instead, the primed EVs showed the immunosuppressive potential by increasing the expression of the anti-inflammatory protein FoxP3 in PBMCs. This may be attributed to the upregulated miRNAs identified in primed EVs in comparison to control EVs (miR-139-5p, miR-140-5p, miR-214-5p). These findings aid in understanding how UCMSC-EVs mediate immunosuppression and support their potential use in treating autoimmune conditions. [Abstract copyright: © 2023. The Author(s).]
Citation
Hyland, M., Mennan, C., Davies, R., Wilson, E. L., Tonge, D. P., Clayton, A., & Kehoe, O. (2023). Extracellular vesicles derived from umbilical cord mesenchymal stromal cells show enhanced anti-inflammatory properties via upregulation of miRNAs after pro-inflammatory priming. Stem Cell Reviews and Reports, 19, 2391–2406. https://doi.org/10.1007/s12015-023-10586-2
Publisher
Springer
Journal
Stem Cell Reviews and Reports
Research Unit
DOI
10.1007/s12015-023-10586-2
PubMed ID
PubMed Central ID
Type
Article
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Description
Series/Report no.
ISSN
2629-3269
EISSN
2629-3277