Publication

Platelet-derived growth factor stimulates osteoprotegerin production in osteoblastic cells

McCarthy, Helen S.
; 
Williams, John H. H.
; 
Davie, Michael W. J.
; 
Marshall, Michael J.
Advisors
Editors
Other Contributors
Affiliation
Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry / University of Chester ; University of Chester ; Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry ; Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry
EPub Date
Publication Date
2008-11-20
Submitted Date
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Biological Sciences
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Abstract
This article discusses how osteoprotegerin (OPG) production by osteoblastic cells was stimulated by platelet-derived growth factor (PDGF) in two human osteosarcoma cell lines (MG63, Saos-2), a mouse pre-osteoblastic cell line (MC3T3-E1) and human bone marrow stromal cells (hMSC) by 152%, 197%, 113% and 45% respectively over 24 h. OPG was measured in the cell culture medium by immunoassay. PDGF isoforms AA, BB and AB show similar stimulation of OPG production. Message for OPG was also increased similarly to the increased secretion into the culture medium. Using specific inhibitors of cell signalling the authors demonstrate that PDGF acts through the PDGF receptor, PKC, PI3K, ERK and P38 and not via NF-kB or JNK. The importance of PDGF in fracture healing suggests a role for OPG production in countering bone resorption during the early phase of this process.
Citation
Journal of Cellular Physiology, 218(2), 2009, pp. 350-354
Publisher
Wiley
Journal
Journal of Cellular Physiology
Research Unit
URI
http://hdl.handle.net/10034/208689
DOI
10.1002/jcp.21600
PubMed ID
PubMed Central ID
Type
Article
Language
en
Description
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Series/Report no.
ISSN
0021-9541
1097-4652
EISSN
ISBN
ISMN
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Test Link
Sponsors
The Bone Disease Foundation
Additional Links
http://doi.wiley.com/10.1002/jcp.21600